Introduction to Toxicology Study Guide

Introduction to Toxicology simulates drug isolation in an authentic forensic screening. Using diatomaceous earth columns, students isolate drugs from simulated urine. They elute the drugs, run them on fluorescent and non-fluorescent chromatographic gels, and use chemical visualization techniques to identify specific drugs. The lab may be done in four standard lab periods or two three-hour lab periods and is easily separated into drug isolation and chromatography sections. Approximately fifty minutes is required for completing the thin layer chromatography and visualization process, which must be done all at one time. Includes forensic scenarios for capturing student interest. Required, not included: fume hood, water bath 65-70C, hair dryer, UV light (356 nm; black light, strong sunlight, plant lights, or a goggle cabinet), UV light short wave (254 nm), and ring stands. Designed for 24 students working in groups of four.

• Authentic forensic toxicology lab: Students simulate real drug isolation from urine using diatomaceous earth columns and forensic screening techniques.
• Chromatography and visualization skills: Learners elute and analyze drugs on fluorescent and non‑fluorescent TLC gels, using chemical and UV visualization to identify substances.
• Flexible, classroom‑ready design: Includes engaging forensic scenarios and can be completed in multiple lab formats; designed for 24 students working in groups of four (some specialized equipment required, not included).